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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 3;41(1):e33–e45. doi: 10.1161/ATVBAHA.120.315154

Figure 3. GPR31 (G-protein–coupled receptor 31) and PAR (protease-activated receptor)-4 form a heterodimer in Chinese hamster ovary (CHO)-K1 recombinant system.

Figure 3.

A and B, Co-immunoprecipitation (IP) experiments using T7-tagged PAR4 and GPR31 transiently transfected in CHO cells. Lysates from T7-PAR4/GPR31 cotransfected cells, co-IP with GPR31 ab, or T7 (PAR4) ab. C, Co-IP experiments using PAR1 and GPR31 transiently transfected in CHO cells were used as control. Western blot analysis performed using PAR4-ab, GPR31-ab, and ATAP2 PAR1-ab as indicated. For deglycosylated IP, washed beads were treated with PNFGase F (endoglycocidase removes N-linked oligosaccharides) enzyme overnight before eluting and running for Western blot analysis.