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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 3;41(1):e33–e45. doi: 10.1161/ATVBAHA.120.315154

Figure 4. Effect of inhibition of GPR31 (G-protein–coupled receptor 31) on thrombin initiated clot retraction.

Figure 4.

A, Platelet-rich plasma was isolated from whole blood and anticoagulated with 4% sodium citrate. Clot retraction was done essentially as described previously.40 Clot retraction was done with samples pretreated with 10 or 30 μM GPR310 (G-protein–coupled receptor 310) or 10 or 30 μM AZD1283 or vehicle, and clot formation was initiated with 3 nmol/L thrombin. Samples were incubated at room temperature over time as indicated and then photographed with a digital camera. B, Two-dimensional retraction clots in A were measured using National Institutes of Health ImageJ and expressed as percent retraction (mean±SD). Statistical significance was determined using ANOVA followed by Bonferonni correction; ****P<0.0001.