Figure 5. GPR310 (G-protein–coupled receptor 310) protects mice from arterial thrombosis.

A and B, Aggregation and ATP secretion was measured in mouse platelet-rich plasma stimulated with 100 μM AYPGKF in the presence of 30 μM GPR310, and ATP release was measured, and aggregation was simultaneously measured. C, Thrombosis was induced with 10% ferric chloride (FeCl₃) application to the carotid artery during which time flow was monitored using Doppler probe. The time to occlusion was determined and plotted for mice treated with 10 mg/kg GPR310 or vehicle (Veh; n=5) delivered subcutaneously 4 h before FeCl₃ injury. Carotid artery occlusion in mice plotted as Kaplan-Meier curves showed significant (P=0.0018, log-rank Mantel-Cox test) protection against FeCl₃-induced carotid artery injury measured over 45 min. D, Ex vivo analysis of mouse platelets collected at 4 h after injection with 10 mg/kg GPR310 or Veh (n=5) stimulated with 50 and 100 μM AYPGKF and 5 and 10 nmol/L thrombin as indicated. Student t test; **P<0.01, ****P<0.0001. E, Mouse tail bleeding was measured 4 h after GPR310 or vehicle sc injection (n=10) as indicated.