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Published in final edited form as: Bone. 2021 Apr 7;148:115946. doi: 10.1016/j.bone.2021.115946

Figure 4. — F4 Legend: A) LAL and β-actin protein expression in murine BMSCs at day 0 and day 7 of osteoblastogenesis, flushed femurs, and testes (n=3). B) Representative images of WT and LAL^−/− calvarial osteoblasts (cOBs) or primary BMSCs treated with lalistat (100 μM) for 7 days during osteoblast differentiation. cOBs were stained for ALP and hematoxylin, while BMSCs were stained for ALP and Von Kossa (VK). Bright field images are also included at 20X magnification (n=3-4). C) BMSCs were cultured under osteogenic differentiation conditions for 0 or 7 days, in the absence (control) or presence of 24 hr treatment with lalistat (100 μM). A) Cells stained with BODIPY 493/503 (green; neutral lipids) and Hoechst 33342 Solution (blue; nuclear material) and visualized by confocal microscopy (n=5). D) Principle component analyses of RNA sequencing from BMSCs isolated from male and female WT or LAL−/− (KO) following 7 days in osteogenic medium (n=2-4). Further analyses of the significantly altered genes in (E) male and (F) female mice.

Figure 4. — F4 Legend: A) LAL and β-actin protein expression in murine BMSCs at day 0 and day 7 of osteoblastogenesis, flushed femurs, and testes (n=3). B) Representative images of WT and LAL^−/− calvarial osteoblasts (cOBs) or primary BMSCs treated with lalistat (100 μM) for 7 days during osteoblast differentiation. cOBs were stained for ALP and hematoxylin, while BMSCs were stained for ALP and Von Kossa (VK). Bright field images are also included at 20X magnification (n=3-4). C) BMSCs were cultured under osteogenic differentiation conditions for 0 or 7 days, in the absence (control) or presence of 24 hr treatment with lalistat (100 μM). A) Cells stained with BODIPY 493/503 (green; neutral lipids) and Hoechst 33342 Solution (blue; nuclear material) and visualized by confocal microscopy (n=5). D) Principle component analyses of RNA sequencing from BMSCs isolated from male and female WT or LAL−/− (KO) following 7 days in osteogenic medium (n=2-4). Further analyses of the significantly altered genes in (E) male and (F) female mice.