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. Author manuscript; available in PMC: 2021 May 10.
Published in final edited form as: Immunity. 2019 Dec 3;52(1):96–108.e9. doi: 10.1016/j.immuni.2019.11.004

Figure 6. IFN-γ contributes to the survival of hepatocytes through upregulation of Bcl-xL.

Figure 6.

(A) Expression of IFN-γR1 on hepatocytes (n = 2). (B) Expression of intracellular Bcl-2, Bcl-xL, and Mcl-1 in hepatocytes of WT and Ifng−/− mice before (naïve) and 18 h after CCl4 injection (n = 2–3). (C) MFI of intracellular Bcl-2, Bcl-xL, and Mcl-1 in hepatocytes of WT and Ifng−/− mice before and 18 h after CCl4 injection. Data were pooled from 2 experiments (n = 2–6). (D) Expression of Bcl-xL in hepatocytes cultured in the presence or absence of CCl4, IFN-γ, or both (n = 3). (E) MFI of Bcl-xL in hepatocytes cultured in the presence or absence of CCl4, IFN-γ, or both (n = 3). (F) Survival of hepatocytes cultured in the presence or absence of CCl4, IFN-γ, or both (n = 4). The percentages of living hepatocytes are shown. Data are representative of 3 (A, D, E, and F) and 2 (B) independent experiments. *p<0.05. **p<0.01, ***p<0.005. Error bars show s.d. See also Figure S6.