Figure 1.

THP1-Xblue (A), RAW-Blue (A, inset) and THP1-XBlue-DefMyD (B) cells were in vitro stimulated for 24 h with PABL or PMBL (10 mg/ml). TNF-α (50 ng/ml) was used as a control of activation in MyD88-deficient cells. LPS (1μg/ml) was used as a control of activation of RAW-Blue cells. SEAP reporter was quantified in culture supernatants by optical density readings at 638 nm using the QUANTI-Blue method. The bars represents Mean ± SEM of triplicate cultures for relative increments of stimulated cells related to non-stimulated ones. Data are representative of three independent experiments. ns, non-significant difference.