Figure 2.

Immunodominant peptides screening via four independent ELISPOT experiments. Splenocytes obtained from humanized mice immunized with inactivated M. tuberculosis in CFA adjuvant (A), lysate of M. tuberculosis in PBS (B), lysate of M. tuberculosis in CFA adjuvant (C), and lysate of M. tuberculosis in IFA adjuvant (D) were stimulated with MP3RT. The SFCs were analyzed by a CTL-S5 Versa ELISPOT Reader. The SI value of 55 peptides was shown as the ratio of SFCs in peptide stimulated cells and medium-stimulated cells. The detailed information of 55 peptides can be found in Table S1 . SI >2 was considered positive and labeled in the figure. The immunodominant peptides included in the MP3RT vaccine were marked using a red text tag.