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. 2021 Apr 26;12:666290. doi: 10.3389/fimmu.2021.666290

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Copyright © 2021 Gong, Liang, Mi, Jia, Xue, Wang, Wang, Zhou, Sun and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and structural prediction of MP3RT vaccine. Six immunodominant peptides were linked with GGGGS or AAY linkers in silico (A). Their antigenic index, surface probability, hydrophobicity, alpha regions, beta regions, and turn regions were assessed using Lasergene Protein software (B). The length and molecular weight of MP3RT were 283aa and 29.70 kDa, respectively (C). The 3D structure of MP3RT was presented in Cartoon, Spacefill, and Surface (C, D). The MP3RT vaccine was prepared in E. coli cells and verified with polyacrylamide gel electrophoresis (E).