Figure 5.

Histopathological characteristics of the lung from mice vaccinated with the MP3RT vaccine. The right lobe of the lung and the rest of the liver obtained from humanized or wild-type mice were used to perform H&E staining and analyzed with software. Each tissue section’s pathological changes were observed using a microscope with original magnification times of 40 × (A) and 100 × (D). The lung lesion area of tissue section obtained from humanized (B) or wild-type mice (C) was marked as red and determined using Image-Pro Plus software. The inflammatory cells of the lung tissue section obtained from humanized (E) or wild-type mice (F) were determined using Image-Pro Plus software. The inflammatory cells were marked in red, alveoli were marked in green, and alveolar walls were marked in yellow. Observations and calculations were done independently by two researchers, and their calculation results were merged. Five tissue sections of each mouse were randomly selected for continuous observation and then took the average value. The results were statistically analyzed with one-way analysis of variance (ANOVA) or Kruskal-Wallis test according to the data normality and homogeneity of variances. All data were shown as mean + SEM (n = 10 in humanized mice vaccinated with PBS and n = 20 in other groups). P < 0.05 was considered significantly different. *P < 0.05; ***P < 0.001; ****P < 0.0001.