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. 2021 Apr 26;12:641224. doi: 10.3389/fimmu.2021.641224

Figure 3.

Figure 3

(A) Monocyte activation state was assessed in whole blood samples of ten healthy individuals stimulated with PBS (control), LPS, MPLA, and mCRP for 15 minutes. An increase in MAN-1 expression demonstrated cell activation. The MFI for MAN-1 of the respective control sample was set as 100. Monocyte activation levels assessed by MAN-1 expression revealed to be highly subset-specific after incubation of the cells with mCRP, whereas stimulation of monocytes with LPS and MPLA led to a general increase in monocyte activation. (B) Whole blood samples of six Wistar rats were analyzed after incubation with PBS (control), LPS, MPLA, and mCRP for 15 minutes, respectively. An increase in CD11b expression demonstrated cell activation. The MFI of the respective control sample was set as 100. Monocyte activation levels assessed by CD11b expression were also highly subset-specific following the pattern seen in human samples, which confirmed the interspecies transferability of the findings. ns, not significant; **p < 0.01; ***p < 0.001; ****p < 0.0001.