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. 2021 May 4;5:237. Originally published 2020 Oct 9. [Version 2] doi: 10.12688/wellcomeopenres.16305.2

Table 1. List of primers and probes sequences used in this study.

Primers and probes sequences used in this study include in-house designed InvA, ttr previously validated for Salmonella detection in food, and TAC- InvA and TAC- ttr used on a well-validated TAC assay as pan Salmonella primers.

Primer name Primer direction Primer code/Probe description
1 INVA Forward 5’-AGCGTACTGGAAAGGGAAAG-3’
2 INVA Reverse 5’-CACCGAAATACCGCCAATAAAG-3’
3 INVA Probe FAM-TTACGGTTCCTTTGACGGTGCGAT-BHQ1
4 ttr Forward 5’-CTCACCAGGAGATTACAACATGG-3’
5 ttr Reverse 5’-AGCTCAGACCAAAAGTGACCATC-3’
6 ttr Probe FAM-CACCGACGGCGAGACCGACTTT-BHQ1
7 InvA-TAC Forward 5’-GGCAATTCGTTATTGGCGATA-3’
8 InvA-TAC Reverse 5’-CACGGTGACAATAGAGAAGACAACA-3’
9 InvA-TAC Probe FAM-CCTGGCGGTGGGTT-MGB
10 ttr-TAC Forward 5’-CTCACCAGGAGATTACAACATGG-3’
11 ttr-TAC Reverse 5’-AGCTCAGACCAAAAGTGACCATC-3’
12 ttr-TAC Probe FAM-CACCGACGGCGAGACCGACTTT-MGB