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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Sep 10;6(10):5941–5958. doi: 10.1021/acsbiomaterials.0c01075

Figure 1. Increased GM-CSF and TGF-β1 loading in the udMP enhanced DC recruitment.

Figure 1.

C57BL/6 mice were injected subcutaneously in the abdominal region with either blank PLGA MPs (BMP), the dMP, or the udMP (n = 4–5). (A) Injection site nodules were excised seven days later, enzymatically digested, and leukocytes analyzed by flow cytometry. (B) Leukocyte frequencies (Ly6G+CD11b+ neutrophils; Ly6G−CD11b+CD11c− macrophages; Ly6G−CD11b+CD11c+ DCs) as a as a percentage of CD45+ live cells. The frequency of DCs recruited to the site of MP injection increased in a bioactive factor dose-dependent manner. P-values (* = ≤ 0.05) were obtained by one-way ANOVA with Tukey’s significance test. Data is represented by mean ± SEM. Full gating scheme – see Supplemental Figure 8.