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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Sep 10;6(10):5941–5958. doi: 10.1021/acsbiomaterials.0c01075

Figure 10. Treg frequency was elevated in all treatment groups compared to untreated mice in dMP-draining lymph nodes, but comparable across groups in pancreatic lymph nodes and spleen.

Figure 10.

Twelve-week-old pre-diabetic NOD mice received aCD3 and dMP treatment at identical time points as in the prevention study (Figure 4A) and were euthanized at 20 weeks of age, prior to the sixth dMP injection. As before, MP injections were administered subcutaneously on the right side of the abdomen, proximal to the pancreas. (A) Ipsilateral dMP-draining lymph nodes (combined inguinal and axillary), pancreatic lymph nodes, and spleen were excised and stained for flow cytometry (n = 4–5/group). Frequency of Foxp3+ Tregs as a percentage of total CD4+ T cells was characterized in (B) dMP-draining lymph nodes, (C) pancreatic lymph nodes, and (D) spleen. P-values (* P ≤ 0.05) were determined by one-way ANOVA with Tukey’s multiple comparison test. Data is represented by mean ± SEM. Full gating scheme – see Supplemental Figure 12.