Skip to main content
. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Sep 10;6(10):5941–5958. doi: 10.1021/acsbiomaterials.0c01075

Figure 2. High dose GM-CSF and TGF-β1 loading in the udMP led to reduced costimulatory marker expression on DCs.

Figure 2.

C57BL/6 mice were injected subcutaneously in the abdominal region with either blank PLGA MPs (BMP), the dMP, or the udMP (n = 4–5). (A) Injection site nodules were excised seven days later, enzymatically digested, and leukocytes analyzed by flow cytometry for CD86 and MHC-II expression on DCs recruited to the site of injection. (B) Frequency of surface marker expression (CD80, CD86, and MHC-II) was characterized on DCs to assess the capacity of the udMP to induce a suppressive phenotype. P-values (* = ≤ 0.05, ** = ≤ 0.01) were obtained by one-way ANOVA with Tukey’s significance test. Data is represented by mean ± SEM. Full gating scheme – see Supplemental Figure 8.