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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Sep 10;6(10):5941–5958. doi: 10.1021/acsbiomaterials.0c01075

Figure 7. Non-depleted T cells displayed a regulatory phenotype.

Figure 7.

(A-B) At ~13 weeks of age, three days after completing a five-day aCD3 treatment regimen, a selection of mice from each group was bled to assess circulating blood lymphocyte frequency and phenotype by flow cytometry (n = 9–10/group). (C) The frequency of Foxp3+ Tregs as a percentage of total CD4+ T cells in blood. Percentage of CD4+ T cells expressing (D) PD-1 or (E) ICOS+ in blood. P-values (* ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001) were determined by one-way ANOVA with Tukey’s multiple comparisons test. Data is represented by mean ± SEM. Full gating scheme – see Supplemental Figure 10.