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. 2021 Apr 4;13(8):11234–11256. doi: 10.18632/aging.202807

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Copyright: © 2021 Zhuo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Determination of optimal cultivation scheme of IhOM-MSCs. (A) We were pretreated OM-MSCs with ischemia/hypoxia at different time gradients, and the RT-qPCR result showed that miR-181a was significantly down-regulated after 36h. (B) Successful mimic of miR-181a in IhOM-MSCs was verified by qPCR. (C–E) Protein and mRNA expression of GRP78 and Bcl-2 in OM-MSCs as determined by western blot and qPCR, respectively. (OM-MSCs were replaced by MSCs in the figure. All data are presented as the mean value ± SD. *p<0.05, **p<0.01).