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. 2021 Apr 18;13(8):11528–11541. doi: 10.18632/aging.202846

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Copyright: © 2021 Shi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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OGD/R treatment increased methylation of the miR-532-5p promoter. (A, B) Methylation-specific PCR (MSP) results show the methylation status of the CpG islands in the miR-532-5p promoter sequence in SH-SY5Y cells treated with OGD/R, 5-Aza or OGD/R + 5-Aza and untreated controls. ‘U’ refers to PCR with primers specific to unmethylated sequence and ‘M’ refers to PCR with primers specific to methylated sequence. (C) RT-qPCR analysis shows the relative expression levels of miR-532-5p in SH-SY5Y cells treated with OGD/R, 5-Aza or OGD/R + 5-Aza and untreated controls. (D) RT-qPCR analysis shows the relative expression levels of CXCL1 mRNA in SH-SY5Y cells treated with OGD/R, 5-Aza or OGD/R + 5-Aza compared to untreated controls. ^**P < 0.01 compared to control; ^#P < 0.05, ^##P < 0.01 compared to OGD/R. All experiments were performed in triplicate.