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. 2021 Apr 4;13(8):11455–11469. doi: 10.18632/aging.202837

Figure 4.

Figure 4

miR-124-3p was the target of HOXA11-AS and FSTL1. (A, B) The targeted association between HOXA11-AS, miR-124-3p and FSTL1 was found in the Starbase database (http://starbase.sysu.edu.cn/). (C, D) Dual-luciferase reporter assay was conducted to verify the targeting relationship between HOXA11-AS and miR-124-3p, miR-124-3p and FSTL1 in SH-SY5Y and BV2 cells. (E–H) RT-PCR and Western blot experiments were adopted to determine FSTL1 mRNA and protein expressions. nsP> 0.05, ** P <0.01, *** P <0.001 (vs.miR-NC group) *P <0.05, *** P <0.001 (vs.vestor group) &&& P <0.001 (vs.miR-124 -3p group).