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[Preprint]. 2021 May 4:2021.05.03.442357. [Version 1] doi: 10.1101/2021.05.03.442357

Figure 4. Prior infection with SARS-CoV-2 protects from lethal SARS-CoV challenge.

Figure 4.

a-c) Ten-week-old female Balb/c mice were previously infected with 106 PFU of SARS-CoV-2 CMA3p20 (blue) or mock (black), monitored for weight loss, and allowed to recover. Twenty-eight days post infection, both groups were challenged with a lethal dose (104 PFU) of mouse-adapted SARS-CoV and evaluated for a) weight loss, b) lethality, and c) disease score. d) Mice were subsequently euthanized at day 2, 4, and 7 and lung tissue examined for viral replication. e) Sera from CMA3p20 infected and SARS-CoV challenged were evaluated for virus neutralization (PRNT50) against SARS-CoV-2 (blue) or SARS-CoV over time (no rechallenge-black, day 2 red, day 4-orange, day 7- green).