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. Author manuscript; available in PMC: 2021 May 10.
Published in final edited form as: Cell Rep. 2016 Jul 28;16(6):1642–1652. doi: 10.1016/j.celrep.2016.06.100

Figure 2. Specification of V2aIN fate requires Chx10.

Figure 2.

(A) Immunohistochemical and in situ hybridization analyses of E12.5 Chx10orJ/+ and Chx10orJ/orJ littermates. The ventral spinal cords are shown. Sox14/Lhx3-expressing V2aINs are reduced in Chx10orJ/orJ spinal cords. While the number of Hb9+/Isl1+ MNs did not significantly change, Hb9+/Isl1+ MNs emigrate abnormally from the spinal cord into the motor axonal track outside of the spinal cord (arrows) in Chx10orJ/orJ mice. The expression of the cholinergic gene VAChT is also increased in the ventral spinal cords of Chx10orJ/orJ mice.

(B) Quantification of the number of V2aINs and MNs in E12.5 mice. The change of the number of V2aINs and MNs in Chx10orJ/orJ spinal cords is relative to their control littermates. The error bars represent the standard error of the mean. **, p<0.01; *, p<0.05; ns, non-significant; n≥5 embryos.