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. Author manuscript; available in PMC: 2021 May 10.
Published in final edited form as: Cell Rep. 2016 Jul 28;16(6):1642–1652. doi: 10.1016/j.celrep.2016.06.100

Figure 5. Chx10 specifies V2a fate through its transcriptional repressor activity and inhibits MN development independently of its transcriptional activity.

Figure 5.

(A) Schematic representation of Chx10 fusions. EnR, Engrailed repressor domain; VP16, VP16 activator domain; HD, homeodomain.

(B) Immunohistochemical and in situ hybridization analyses in chick spinal cords electroporated (+ side) with EnR-Chx10 or with VP16-Chx10. EnR-Chx10 promotes the expression of V2aIN markers and inhibits MN specification, whereas VP16-Chx10 inhibits generation of both V2aINs and MNs. Ectopic V2aINs in the dorsal spinal cord and the MN area are marked with brackets.