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. 2021 Apr 26;12:657486. doi: 10.3389/fphar.2021.657486

FIGURE 1.

FIGURE 1

Caspase-11-gasdermin D medicated pyroptosis signaling is involved in the pathogenesis of atherosclerosis. (A) Histogram showing numbers of different expression genes (DEGs) of included datasets in GEO database. DEGs with the cut-off criteria of −log10 False Discovery Rate (−logFDR) > 2 and |log2 fold change (FC)| > 2 were colored in red for up-regulated gens while in blue for down-regulated genes. (B) Venn diagrams depict 24 genes with consistent pattern of expression across four available transcriptomic datasets from aortic arterial tissue from C57BL/6J or ApoE−/− mice fed with normal chow diet or HFHC in the GEO database. (C) Heatmap of 24 DEGs in four datasets of mouse aortas from normal chow diet or HFHC diet. The abscisa represents different GEO datasets and samples, and the ordinate represents different genes. The red boxes indicate up-regulated genes, and the blue boxes indicate down-regulated genes. (D) GO term enrichment analysis illustrating number of significantly up-regulated in the most affected up-regulated genes common in HFHC diet mice aortas compared to the controls. (E) Principal component analysis of bulk RNA-seq data in peritoneal macrophages primed with LPS followed by 16 h of ox-LDL stimulation of control and LDL-treated macrophages. (F) Volcano plot indicating transcriptomic changes in control and LDL-treated macrophages. The red dots indicate up-regulated genes, and the blue dots indicate down-regulated genes. (G) Heatmap with differentially expressed genes in control and LDL-treated macrophages. DEGs with the cut-off criteria of −log10 False Discovery Rate (−logFDR) > 2 and |log2 fold change (FC)| > 2 were colored in red for up-regulated gens while in blue for down-regulated genes. The abscisa represents samples, and the ordinate represents different genes. The red boxes indicate up-regulated genes, and the blue boxes indicate down-regulated genes. (H) Gene Set Enrichment Analysis in control and LDL-treated macrophages. GEO, Gene Expression Omnibus; HFHC, high fat high cholesterol; GO term, Gene Ontology term; PCA, Principal component analysis.