| Antibodies |
| Biotin anti-human CD2 |
BioLegend |
Cat# 300204, RRID:AB_314028 |
| Biotin anti-human CD3 |
BioLegend |
Cat# 300304, RRID:AB_314040 |
| Biotin anti-human CD4 |
BioLegend |
Cat# 300504, RRID:AB_314072 |
| Biotin anti-human CD7 |
Thermo Fisher Scientific |
Cat# 13-0079-82, RRID:AB_891490 |
| Biotin anti-human CD8a |
BioLegend |
Cat# 301004, RRID:AB_314122 |
| Biotin anti-human CD10 |
Thermo Fisher Scientific |
Cat# 13-0108-82, RRID:AB_763433 |
| Biotin anti-human CD11b |
BioLegend |
Cat# 301304, RRID:AB_314156 |
| Biotin anti-human CD14 |
BioLegend |
Cat# 325624, RRID:AB_2074052 |
| Biotin anti-human CD19 |
BioLegend |
Cat# 302204, RRID:AB_314234 |
| Biotin anti-human CD20 |
Thermo Fisher Scientific |
Cat# 13-0209-82, RRID:AB_657690 |
| Biotin anti-human CD56 |
BioLegend |
Cat# 318320, RRID:AB_893390 |
| Biotin anti-human CD235ab |
BioLegend |
Cat# 306618, RRID:AB_2565773 |
| APC anti-human CD34 |
BD Biosciences |
Cat# 340667, RRID:AB_400531 |
| PE/Cy7 anti-human CD38 |
BioLegend |
Cat# 303516, RRID:AB_2072782 |
| FITC anti-human CD90 |
BD Biosciences |
Cat# 555595, RRID:AB_395969 |
| PB anti-human CD45RA |
Thermo Fisher Scientific |
Cat# MHCD45RA28, RRID:AB_10371901 |
| PE anti-human CD123 |
BD Biosciences |
Cat# 554529, RRID:AB_395457 |
| BV605 anti-human Ki-67 |
BioLegend |
Cat# 350522, RRID:AB_2563863 |
| FITC anti-mouse Ly-6G |
BioLegend |
Cat# 127606, RRID:AB_1236494 |
| PE anti-mouse/human CD11b |
BioLegend |
Cat# 101208, RRID:AB_312791 |
| PerCP/Cy5.5 anti-mouse CD3e |
BD Biosciences |
Cat# 551163, RRID:AB_394082 |
| BV421 anti-mouse CD115 |
BioLegend |
Cat# 135513, RRID:AB_2562667 |
| BV605 anti-mouse Ly-6C |
BioLegend |
Cat# 128035, RRID:AB_2562352 |
| BV711 anti-mouse CD8a |
BioLegend |
Cat# 100748, RRID:AB_2562100 |
| AF700 anti-mouse CD4 |
BioLegend |
Cat# 100430, RRID:AB_493699 |
| APC anti-mouse CD45.2 |
BioLegend |
Cat# 109814, RRID:AB_389211 |
| PE/Cy7 anti-mouse CD45.1 |
BD Biosciences |
Cat# 560578, RRID:AB_1727488 |
| APC/Cy7 anti-mouse CD19 |
BioLegend |
Cat# 115529, RRID:AB_830706 |
| APC/Cy7 anti-mouse/human B220 |
BioLegend |
Cat# 103224, RRID:AB_313007 |
| APC/Cy7 anti-mouse NK1.1 |
BioLegend |
Cat# 108724, RRID:AB_830871 |
| Biotin anti-mouse CD3 |
BioLegend |
Cat# 100304, RRID:AB_312669 |
| Biotin anti-mouse CD4 |
BioLegend |
Cat# 100404, RRID:AB_312689 |
| Biotin anti-mouse CD8a |
BioLegend |
Cat# 100704, RRID:AB_312743 |
| Biotin anti-mouse CD49b |
BioLegend |
Cat# 103522, RRID:AB_2566366 |
| Biotin anti-mouse CD90.2 |
BioLegend |
Cat# 105304, RRID:AB_313175 |
| Biotin anti-mouse CD19 |
BioLegend |
Cat# 115504, RRID:AB_313639 |
| Biotin anti-mouse B220 |
BioLegend |
Cat# 103204, RRID:AB_312989 |
| Biotin anti-mouse NK1.1 |
BioLegend |
Cat# 108704, RRID:AB_313391 |
| Biotin anti-mouse TER119 |
BioLegend |
Cat# 116204, RRID:AB_313705 |
| Biotin anti-mouse CD11b |
BioLegend |
Cat# 101204, RRID:AB_312787 |
| Biotin anti-mouse CD11c |
BioLegend |
Cat# 117304, RRID:AB_313773 |
| Biotin anti-mouse Gr-1 |
BioLegend |
Cat# 108404, RRID:AB_313369 |
| FITC anti-mouse CD34 |
BD Biosciences |
Cat# 553733, RRID:AB_395017 |
| PE anti-mouse CD45.2 |
BioLegend |
Cat# 109808, RRID:AB_313445 |
| PerCP/Cy5.5 anti-mouse CD150 |
BioLegend |
Cat# 115922, RRID:AB_2303663 |
| PE/Cy7 anti-mouse c-kit |
BioLegend |
Cat# 105814, RRID:AB_313223 |
| AF700 anti-mouse CD48 |
BioLegend |
Cat# 103426, RRID:AB_10612755 |
| BV421 anti-mouse CD45.1 |
BioLegend |
Cat# 110732, RRID:AB_2562563 |
| BV605 anti-mouse Sca-1 |
BioLegend |
Cat# 103244, RRID:AB_2563312 |
| BV711 anti-mouse CD16/32 |
BioLegend |
Cat# 101337, RRID:AB_2565637 |
| PE anti-mouse CD8a |
BioLegend |
Cat# 100708, RRID:AB_312747 |
| PerCP/Cy5.5 anti-mouse CD45.1 |
BioLegend |
Cat# 110726, RRID:AB_893345 |
| PacificBlue anti-mouse CD4 |
BioLegend |
Cat#100428, RRID:AB_493647 |
| BV605 anti-mouse CD115 |
BioLegend |
Cat#135517, RRID:AB_2562760 |
| BV711 anti-mouse/human CD11b |
BioLegend |
Cat#101242, RRID:AB_2563310 |
| APC anti-mouse CD3 |
BioLegend |
Cat#100236, RRID:AB_2561456 |
| APC anti-mouse CD90 |
BioLegend |
Cat#140312, RRID:AB_10640728 |
| APC anti-mouse TCRβ |
BioLegend |
Cat#109212, RRID:AB_3135435 |
| APC anti-mouse CD19 |
BioLegend |
Cat#152410, RRID:AB_2629839 |
| AF700 anti-mouse CD45.2 |
BioLegend |
Cat#109822, RRID:AB_493731 |
| APC/Cy7 anti-mouse Ly6C |
BioLegend |
Cat#128026, RRID:AB_10640120 |
| PE anti-mouse/human B220 |
BioLegend |
Cat#103208, RRID:AB_312993 |
| PE anti-mouse CD19 |
BioLegend |
Cat#115508, RRID:AB_313643 |
| PE anti-mouse CD49b |
BioLegend |
Cat#108908, RRID:AB_313415 |
| PE anti-mouse Ter119 |
BioLegend |
Cat#116208, RRID:AB_313709 |
| PE anti-mouse CD90.2 |
BioLegend |
Cat#105308, RRID:AB_313179 |
| PE anti-mouse CD11b |
BioLegend |
Cat#101208, RRID:AB_312791 |
| PE anti-mouse CD11c |
BioLegend |
Cat#117308, RRID:AB_313777 |
| PE anti-mouse Ly6G |
BioLegend |
Cat#127608, RRID:AB_1186099 |
| PE anti-mouse IL7Rα |
BioLegend |
Cat#135010, RRID:AB_1937251 |
| PerCP/Cy5.5 anti-mouse CD16/32 |
BioLegend |
Cat#101324, RRID:AB_1877267 |
| BV421 anti-mouse CD115 |
BioLegend |
Cat#135513, RRID:AB_2562667 |
| BV605 anti-mouse CD150 |
BioLegend |
Cat#115927, RRID:AB_11204248 |
| BV711 anti-mouse CD45 |
BioLegend |
Cat#103147, RRID:AB_2564383 |
| APC/Cy7 anti-mouse Sca-1 |
BioLegend |
Cat#108126, RRID:AB_10645327 |
| Biological Samples |
| Healthy human bone marrow |
HemaCare BioResearch Products |
Cat# BM009C-2 |
| Healthy human bone marrow |
AcceGen Biotech |
Cat# ABC-TC3122 |
| Critical Commercial Assays |
| APC BrdU Flow Kit |
BD Biosciences |
Cat# 552598 |
| Experimental Models:
Organisms/Strains |
| C57BL/6J mice |
The Jackson Laboratory |
Cat# 000664 |
| B6.129P2-Apoetm1Unc/J mice |
The Jackson Laboratory |
Cat# 002052 |
| B6(Cg)-Tet2tm1.2Rao/J mice |
The Jackson Laboratory |
Cat# 023359 |
| B6.129S7-Ldlrtm1Her/J mice |
The Jackson Laboratory |
Cat# 002207 |
| B6.SJL-Ptprca Pepcb/BoyJ
mice |
The Jackson Laboratory |
Cat# 002014 |
| Software and Algorithms |
| FlowJo 10.6 |
BD |
https://www.flowjo.com/ |
| Prism 8 |
GraphPad Software |
https://www.graphpad.com/ |
| Mathematica, Matlab, R and Python code
producing analyses in this paper |
This paper |
https://github.com/AlexHeyde/clonal-hematopoiesis |
