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. Author manuscript; available in PMC: 2021 May 10.
Published in final edited form as: Sci Immunol. 2020 Jun 19;5(48):eabb1025. doi: 10.1126/sciimmunol.abb1025

Fig. 5. 3M-052 adjuvant formulated in PLGA NP’s with or without GLA significantly improves Ab dependent cellular cytotoxicity (ADCC) as well as avidity of Ab responses durable in serum upto ~ 1 year in comparison with the clinically used alum adjuvant.

Fig. 5.

ADCC activity was assayed using an established Granzyme Toxi Lux or GTL assay. Avidity of polyclonal sera was assayed on purified serum immunoglobulin (IgG) using a BIAcore surface plasmon resonance (SPR) assay. (A) Line graphs show kinetics of ADCC titers observed in the study. (B) Scatter plots display an early peak ADCC activity observed after the 2rd vaccination in the study at week 8. (C) Scatter plots show persistent ADCC activity at termination of the study at week 70. (D) Scatter plots display avidity analysis at termination (week 70) against homologous 1086.C Env gp140, 120 and V1V2antigens. Avidity is calculated by dividing binding response units (BRU) by inverse of the dissociation rate constant or kOFF values. (E) Scatter plots display kOFF or dissociation rates against homologous, consensus and heterologous antigens. (F) Scatter plots display Avidity Scores against homologous, consensus and heterologous antigens. Statistical significance of differences in magnitude of immune responses was tested using a non-parametric Wilcoxon rank-sum test (also known as Mann-Whitney U test) using the R software version 3.6.1. . **** p<0.0001: *** p<0.001: ** p<0.01, *p<0.05.