Fig 2.

In vitro transfer of PtdIns4P from donor to acceptor vesicles in the presence of rEhLTP1 (A,B) and rEhLTP3 (C,D). Donor vesicles (DV) (1.5 μl) containing PtdIns4P were incubated in separate reaction without acceptor vesicles (AV) and recombinant proteins (“DV ONLY”), with 0.5 μg of either rEhLTP1 or rEhLTP3 (“DV+LTP”), with 3 μl of AV (“DV+AV”), or with 3 μl of AV and 0.5 μg of either rEhLTP1 or rEhLTP3 (“DV+AV+LTP”) at room temperature. Fluorescence units were measured after 30 and 60 min of incubation. The percentage of transfer of PtdIns4P (B, D) were calculated from (A) and (C), respectively as described in Methods section. The experiments were conducted in duplicates three times (n = 3 with error bars indicating standard deviations). Statistical comparisons were made by Student’s t-test (*P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005). The y axis represents arbitrary fluorescence units.