FIG. 3.
Coupling of TSHRs to the Gs-dependent signaling pathway in cultured pituitary cells. (A) Time course of TSH-induced cAMP release in pituitary cells from males. (B) Concentration dependence of TSH on intracellular cAMP accumulation in pituitary cells from females. Before TSH application, medium of cells in static cultures was replaced with 0.1% BSA containing medium 199 supplemented with 1 mM IBMX. At the end of incubation with TSH, medium was collected to measure released cAMP by radioimmunoassay, or cells were lysed, and content collected to measure cAMP intracellular accumulation by ELISA as described in the Materials and Methods section. BSA, bovine serum albumin; ELISA, enzyme-linked immunosorbent assay. Color images are available online.