Fig. 1 |. H1 depletion in the haematopoietic compartment results in reduced numbers of terminally differentiated lymphocytes in immune tissues.

a, In silico analysis of H1 expression using mouse FANTOM5⁶ cap analysis gene expression–sequencing (CAGE-seq) data. Total H1 was calculated as the sum of the relative log expression of normalized CAGE-seq reads mapping to the unique 5′ RNA sequences of the 11 H1 subtype transcripts. Shown is the percentage of total H1 corresponding to subtypes H1C, H1D and H1E as a function of the number of H1 subtypes expressed in each tissue. CMP, common myeloid progenitors; ES, embryonic stem cell; GMP, granulocyte–macrophage progenitors; HSC, haematopoietic stem cells; MEG, megakaryocytes. b, Number of B cells, CD4⁺ and CD8⁺ T cells in spleens (n = 6) of wild-type and H1cTKO;Vav-iCre mice, as measured by flow cytometry. c, The frequency of the indicated immature and mature T cell populations in the thymus of wild-type (n = 4) and H1cTKO;Vav-iCre (n = 4) mice was measured by flow cytometry. Absolute cell number was calculated by multiplying the relative frequency of each population by the mean thymus cellularity for wild-type and H1cTKO;Vav-iCre mice. Data are mean ± s.d.; unpaired t-test; *P ≤ 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.