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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Cancer Discov. 2020 Oct 6;11(2):480–499. doi: 10.1158/2159-8290.CD-20-0331

Figure 5. YTHDF2 stabilizes MYC mRNA in GSCs but not in NSCs. See also Figure S6.

Figure 5.

A, B, mRNA levels of MYC and MYC target genes (IGFBP3, VEGFA, HK2, and ENO1) assessed by quantitative RT-PCR following MYC knockdown with two, non-overlapping shRNAs or a non-targeting control sequence in (A) 387 and (B) 4121 GSCs. *, p<0.05, **, p < 0.01. Error bars show standard deviation. C and D, Cell viability measured in sgYTHDF2 #1-, sgYTHDF2 #2- or sgCONT-transduced GSCs in the presence or absence of MYC overexpression. **, p < 0.01. Error bars show standard deviation. E and F, Relative mRNA levels of YTHDF2 target genes normalized to 18S mRNA levels in GSCs transduced with sgYTHDF2 #2 or sgCONT, in the presence or absence of exogenous MYC overexpression. *, p < 0.05, **, p < 0.01. Error bars show standard deviation. G and H, Relative YTHDF2 and MYC mRNA level normalized to 18S mRNA levels in NSC11 and 4121 GSCs transfected with siRNA against YTHDF2 (siYTHDF2) or scrambled control siRNA (siCONT).**, p < 0.01. Error bars show standard deviation. I, Representative western blots showing the protein levels of YTHDF2 and MYC normalized to GAPDH level in NSC11 and 4121 GSCs transfected with siYTHDF2 or siCONT at 72 hours and 96 hours post transfection. J, MYC mRNA assessed over a time course following actinomycin-D treatment in siYTHDF2- or siCONT-transduced NSCs and GSCs. K, Cell viability of NSCs and GSCs transfected with siYTHDF2 or siCONT over a time course up to 96 hours post-transfection. *, p < 0.05, **, p < 0.01. Error bars show standard deviation.