Table II.
Immunologic characteristics of allergic and nonallergic subjects (n = 83)
| Nonallergic | Allergic | P value | AUC ROC (95% CI) | |||
|---|---|---|---|---|---|---|
| Hazelnut allergy | N = 57 | N = 26 | ||||
| SPT weal diameter (mm) | 0.0 (0-4) | 10.0 (6-14) | <.001 | 0.8721 | 0.7984 | 0.9458 |
| Specific IgE (kU/L) | ||||||
| Hazelnut | 0.64 (0.1-3.8) | 6.45 (2.5-18.5) | <.001 | 0.7763 | 0.6764 | 0.8762 |
| Cor a 1 | 0.01 (0.0-2.1) | 0.57 (0.0-10.7) | .026 | 0.6495 | 0.5205 | 0.7785 |
| Cor a 8 | 0.01 (0.0-0.0) | 0.02 (0.0-0.1) | .058 | 0.6082 | 0.4784 | 0.7380 |
| Cor a 9 | 0.13 (0.0-0.9) | 4.20 (0.3-8.8) | <.001 | 0.7390 | 0.6173 | 0.8608 |
| Cor a 14 | 0.02 (0.0-0.1) | 3.27 (0.3-16.0) | <.001 | 0.8659 | 0.7717 | 0.9600 |
| Basophil activation test (%CD63+ basophils) | ||||||
| Hazelnut 113.64 ng/mL | 0.0 (0-1) | 7.7 (0-36) | <.001 | 0.7510 | 0.6301 | 0.8719 |
| Hazelnut 22.73 ng/mL | 0.0 (0-0) | 19.8 (8-52) | <.001 | 0.8556 | 0.7558 | 0.9554 |
| Hazelnut 4.545 ng/mL | 0.0 (0-0) | 9.6 (1-31) | <.001 | 0.8691 | 0.7831 | 0.9551 |
| Hazelnut 0.9091 ng/mL | 0.0 (0-0) | 3.2 (0-26) | <.001 | 0.8424 | 0.7519 | 0.9330 |
| Cashew nut allergy | N = 48 | N = 35 | ||||
| SPT weal diameter (mm) | 0.0 (0-2) | 12.0 (9-15) | <.001 | 0.9762 | 0.9422 | 1.0000 |
| Specific IgE to cashew (kU/L) | 0.19 (0.0-0.7) | 4.15 (1.1-10.8) | <.001 | 0.8867 | 0.8148 | 0.9587 |
| Specific IgE to Ana o 3 (kU/L) | 0.01 (0.0-0.1) | 3.89 (0.9-10.7) | <.001 | 0.9737 | 0.9417 | 1.0000 |
| Basophil activation test (%CD63+ basophils) | ||||||
| Cashew 113.64 ng/mL | 0.0 (0-1) | 12. (2-45) | <.001 | 0.8673 | 0.7798 | 0.9548 |
| Cashew 22.73 ng/mL | 0.0 (0-1) | 14.4 (3-51) | <.001 | 0.8750 | 0.7939 | 0.9561 |
| Cashew 4.545 ng/mL | 0.0 (0-1) | 13.3 (1-34) | <.001 | 0.8452 | 0.7577 | 0.9328 |
| Cashew 0.9091 ng/mL | 0.0 (0-1) | 1.9 (0-17) | .001 | 0.7036 | 0.5892 | 0.8180 |
| Almond allergy | N = 79 | N = 3 | ||||
| SPT weal diameter (mm) | 0.0 (0-2) | 8.0 (3-12) | .005 | 0.8945 | 0.7028 | 1.0000 |
| Specific IgE to almond (kU/L) | 0.20 (0.1-1.3) | 1.64 (1.5-2.8) | .065 | 0.8143 | 0.7250 | 0.9037 |
| Basophil activation test (%CD63+ basophils) | ||||||
| Almond 113.64 ng/mL | 0.1 (0-1) | 14.5 (1-38) | .013 | 0.9125 | 0.7895 | 1.0000 |
| Almond 22.73 ng/mL | 0.2 (0-1) | 8.6 (0-44) | .085 | 0.7833 | 0.4402 | 1.0000 |
| Almond 4.545 ng/mL | 0.1 (0-1) | 15.3 (1-17) | .018 | 0.8833 | 0.6981 | 1.0000 |
| Almond 0.9091 ng/mL | 0.0 (0-1) | 0.1 (0-22) | .396 | 0.6292 | 0.2427 | 1.0000 |
| Sesame seed allergy | N = 71 | N = 12 | ||||
| SPT weal diameter (mm) | 0.0 (0-1) | 12.5 (8-21) | <.001 | 0.9137 | 0.7969 | 1.0000 |
| Specific IgE to sesame (kU/L) | 0.30 (0.1-2.3) | 3.10 (1.6-29.1) | <.001 | 0.8173 | 0.7140 | 0.9205 |
| Basophil activation test (%CD63+ basophils) | ||||||
| Sesame 113.64 ng/mL | 0.0 (0-0) | 27.7 (11-79) | <.001 | 0.9337 | 0.8109 | 1.0000 |
| Sesame 22.73 ng/mL | 0.1 (0-1) | 26.6 (1-48) | <.001 | 0.8504 | 0.7004 | 1.0000 |
| Sesame 4.545 ng/mL | 0.0 (0-1) | 2.7 (0-16) | .003 | 0.7359 | 0.5552 | 0.9166 |
| Sesame 0.9091 ng/mL | 0.0 (0-1) | 0.3 (0-3) | .306 | 0.5874 | 0.3961 | 0.7788 |
| Peanut allergy | N = 35 | N = 48 | ||||
| SPT weal diameter (mm) | 0.0 (0-3) | 10.5 (9-15) | <.001 | 0.9314 | 0.8734 | 0.9893 |
| Specific IgE (kU/L) | ||||||
| Peanut | 0.35 (0.1-2.1) | 14.60 (3.4-50.9) | <.001 | 0.8984 | 0.8328 | 0.9639 |
| Ara h 1 | 0.01 (0.0-0.1) | 0.72 (0.0-11.9) | <.001 | 0.7696 | 0.6686 | 0.8706 |
| Ara h 2 | 0.01 (0.0-0.1) | 10.80 (1.6-33.6) | <.001 | 0.9536 | 0.9033 | 1.0000 |
| Ara h 3 | 0.03 (0.0-0.1) | 0.13 (0.0-1.6) | .028 | 0.6222 | 0.4993 | 0.7451 |
| Ara h 8 | 0.03 (0.0-1.0) | 0.01 (0.0-1.3) | .406 | 0.5585 | 0.4352 | 0.6817 |
| Ara h 9 | 0.01 (0.0-0.1) | 0.01 (0.0-0.0) | .155 | 0.3768 | 0.2588 | 0.4948 |
| Basophil activation test (%CD63+ basophils) | ||||||
| Peanut 22.73 ng/mL | 0.3 (0-1) | 37.3 (10-68) | <.001 | 0.8655 | 0.7862 | 0.9447 |
| Peanut 4.55 ng/mL | 0.0 (0-0) | 28.7 (2-53) | <.001 | 0.8595 | 0.7810 | 0.9381 |
| Peanut 0.909 ng/mL | 0.0 (0-0) | 6.1 (0-24) | <.001 | 0.7595 | 0.6621 | 0.8570 |
| Ara h 1 22.724 ng/mL | 0.0 (0-1) | 24.0 (0-50) | <.001 | 0.7753 | 0.6780 | 0.8726 |
| Ara h 1 4.545 ng/mL | 0.0 (0-1) | 8.6 (0-36) | <.001 | 0.7762 | 0.6807 | 0.8717 |
| Ara h 1 0.9091 ng/mL | 0.0 (0-0) | 1.0 (0-11) | <.001 | 0.7173 | 0.6119 | 0.8226 |
| Ara h 2 4.55 ng/mL | 0.0 (0-0) | 20.5 (3-53) | <.001 | 0.8696 | 0.7891 | 0.9502 |
| Ara h 2 0.91 ng/mL | 0.0 (0-0) | 20.7 (5-47) | <.001 | 0.8524 | 0.7686 | 0.9362 |
| Ara h 2 0.182 ng/mL | 0.0 (0-1) | 17.7 (2-50) | <.001 | 0.8256 | 0.7376 | 0.9136 |
| Ara h 6 4.55 ng/mL | 0.0 (0-0) | 27.1 (1-67) | <.001 | 0.8250 | 0.7373 | 0.9127 |
| Ara h 6 0.91 ng/mL | 0.0 (0-0) | 14.0 (0-48) | <.001 | 0.8295 | 0.7459 | 0.9130 |
| Ara h 6 0.182 ng/mL | 0.0 (0-0) | 2.1 (0-61) | <.001 | 0.7137 | 0.6084 | 0.8190 |
AUC, Area under the curve; CI, confidence interval; ROC, receiver operating characteristic; SPT, skin prick testing.
Median and interquartile range are represented. Subjects with nonresponder basophils were excluded. P < .05 were considered significant and marked in bold.