Table III.
Cutoffs for the basophil activation test to different nuts and their diagnostic performance (n = 83, nonresponders were excluded)
| Allergen | Cutoff | Sensitivity (95% CI) | Specificity (95% CI) | Positive predictive value (95% CI) | Negative predictive value (95% CI) |
|---|---|---|---|---|---|
| BAT to hazelnut | 60.98 | 15.38 (4.4-34.9) | 100.00 (93.7-100) | 100.00 (39.8-100) | 72.15 (60.9-81.7) |
| 0.924 | 80.77 (60.7-93.5) | 87.72 (76.3-94.9) | 75 (55.1-89.3) | 90.91 (80.0-97.0) | |
| 0.13 | 92.31 (74.9-99.1) | 66.67 (52.9-78.6) | 55.81 (39.9-70.9) | 95.00 (83.1-99.4) | |
| BAT to cashew | 25.21 | 42.86 (26.3-60.7) | 100 (92.6-100) | 100 (78.2-100) | 70.6 (58.3-81.0) |
| 1.79 | 82.86 (66.4-93.4) | 87.5 (74.8-95.3) | 82.86 (66.4-93.4) | 87.5 (74.8-95.3) | |
| 0.36 | 88.57 (73.3-96.8) | 70.83 (55.9-83.1) | 68.89 (53.4-81.8) | 89.5 (75.2-97.1) | |
| BAT to sesame | 16.11 | 66.67 (34.9-90.1) | 100 (94.9-100) | 100 (63.1-100) | 94.67 (86.9-98.5) |
| 8.15 | 91.67 (61.5-99.8) | 98.59 (92.4-100) | 91.67 (61.5-99.8) | 98.59 (92.4-100) | |
| 14.26 | 75 (42.8-94.5) | 98.59 (92.4-100) | 90 (55.6-99.8) | 95.89 (88.5-99.1) | |
| BAT to almond | 37.57 | 33.33 (0.8-90.6) | 100 (95.5-100) | 100 (2.5-100) | 97.56 (91.5-99.7) |
| 0.825 | 100 (29.2-100) | 80 (69.6-88.1) | 15.79 (3.4-39.6) | 100 (94.4-100) | |
| 18.63 | 33.33 (0.8-90.6) | 93.75 (86.0-97.9) | 16.67 (0.4-64.1) | 97.4 (90.9-99.7) | |
| BAT to peanut | 42.11 | 45.83 (31.4-60.8) | 97.14 (85.1-99.9) | 95.65 (78.1-99.9) | 56.67 (43.2-69.4) |
| 4.717 | 81.25 (67.4-91.1) | 85.7 (69.7-95.2) | 88.64 (75.4-96.2) | 76.92 (60.7-88.9) | |
| 0.124 | 93.75 (82.8-98.7) | 37.14 (21.5-55.1) | 67.16 (54.6-78.2) | 81.25 (54.4-96.0) | |
| BAT to Ara h 1 | 16.02 | 39.58 (25.8-54.7) | 97.14 (85.1-99.9) | 95.00 (75.1-99.9) | 53.97 (40.9-66.6) |
| 0.82 | 64.58 (49.5-77.8) | 85.71 (69.7-95.2) | 86.11 (70.5-95.3) | 63.83 (48.5-77.3) | |
| 0.005 | 79.17 (65.0-89.5) | 60.00 (42.1-76.1) | 73.08 (59.0-84.4) | 67.74 (48.6-83.3) | |
| BAT to Ara h 2 | 2.264 | 79.17 (65.0-89.5) | 94.29 (80.8-99.3) | 95 (83.1-99.4) | 76.74 (61.4-88.2) |
| 0.57 | 83.33 (69.8-92.5) | 91.43 (76.9-98.2) | 93.02 (80.9-98.5) | 80 (64.4-91.0) | |
| 0.375 | 85.42 (72.2-93.9) | 85.71 (69.7-95.2) | 89.13 (76.4-96.4) | 81.08 (64.8-92.0) | |
| BAT to Ara h 6 | 26.71 | 43.75 (29.5-58.8) | 97.14 (85.1-99.9) | 95.45 (77.2-99.9) | 55.74 (42.5-68.5) |
| 0.96 | 72.92 (58.2-84.7) | 88.57 (73.3-96.8) | 89.74 (75.8-97.1) | 70.45 (54.8-83.2) | |
| 0.325 | 79.17 (65.0-89.5) | 74.29 (56.7-87.5) | 80.85 (66.7-90.9) | 72.22 (54.8-85.8) |
BAT, Basophil activation test; CI, confidence interval.
Optimal concentrations of allergen were 22.73 ng/mL for peanut, 45.45 ng/mL for Ara h 1, 4.55 ng/mL for Ara h 2, 0.91 ng/mL for Ara h 6, 4.545 ng/mL for hazelnut, 22.73 ng/mL for cashew, 113.64 ng/mL for almond, and 113.64 ng/mL for sesame.