Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-HA (mouse, monoclonal) |
Santa Cruz | sc-7392 RRID:AB_627809 |
IF(1:1,000) |
| Antibody | Anti-HA (rabbit, monoclonal) |
Cell Signaling Technology | 3724 RRID:AB_1549585 |
IF(1:2,000) only used in Figure 1—figure supplements 1D and 3D |
| Antibody | Anti-DDDDK (anti-FLAG) (rabbit, polyclonal) | MBL | PM020 RRID:AB_591224 |
IF(1:500) |
| Antibody | Anti-V5 (chicken, polyclonal) | Abcam | ab9113 RRID:AB_307022 |
IF(1:1000) |
| Antibody | Anti-p60 (rabbit, monoclonal) |
Abcam | ab109442 RRID:AB_10861771 |
IF(1:400) |
| Antibody | Anti-HIRA (mouse, monoclonal) |
Active Motif | WC119.2H11 RRID:AB_10715607 |
IF(1:200) |
| Antibody | Anti-HIRA (rabbit, monoclonal) |
Abcam | ab129169 RRID:AB_11140220 |
WB(1:500) |
| Antibody | Anti-p60 (rabbit, monoclonal) |
Abcam | ab109442 RRID:AB_10861771 |
WB(1:400) |
| Antibody | Anti-H3 (mouse, monoclonal) | MBL | MABI0301 RRID:AB_11142498 |
WB(1:1000) |
| Antibody | Anti-SRCAP (rabbit, polyclonal) |
Kerafast | ESL103 | WB(1:1000) |
| Antibody | Anti-TIP60 (mouse, monoclonal) |
Santa Cruz | sc-166323 RRID:AB_2296327 |
WB(1:50) |
| Antibody | Anti-ANP32E (rabbit, polyclonal) | MyBioSource | MBS9214243 | WB(1:1000) |
| Antibody | Anti-GAPDH (mouse, monoclonal) | MBL | ML171-3 RRID:AB_10597731 |
WB(3:1000) |
| Sequence-based reagent | GAPDH_F | This paper | PCR primers | AAAGGGTGCAGCTGAGCTAG |
| Sequence-based reagent | GAPDH_R | This paper | PCR primers | TACGAAGCCCTTCCAGGAGA |
| Sequence-based reagent | LINC02199_F | This paper | PCR primers | CCGGTGTCAAATGTCACAATGAA |
| Sequence-based reagent | LINC02199_R | Thermo Fisher | PCR primers | GGGGTTTTGAGGATTCCAAAGTG |
| Sequence-based reagent | si_p60 gene | This paper | siRNA | AAUGAUAACAAGGAGCCGGAGdTdT |
| Sequence-based reagent | si_p150 gene | This paper | siRNA | CUGUCAUGUGGGUUCUGACdTdT |
| Sequence-based reagent | ON-TARGET SMARTpool siRNA (HIRA gene) | Dharmacon | siRNA | L-013610-00-0005 |
| Cell line (Homo sapiens) |
HeLa | Peter R Cook | N/A | A human cervical cancer cell line (female origin) |
| Cell line (Homo sapiens) |
SF8628 | Merck | SCC127 RRID:CVCL_IT46 |
Human DIPG H3.3-K27M Cell Line |