Table 2. qRT-PCR primer sequences for zebrafish genes.
F-lef1 | AATGATCCCGTTCAAAGACG |
---|---|
R-lef1 | CGCTAAGTCTCCCTCCTCCT |
F-shha | CCACTACGAGGGAAGAGCTG |
R-shha | GAGCAATGAATGTGGGCTTT |
F-aldh1a2 | AACCACTGAACACGGACCTC |
R-aldh1a2 | CTCCAGTTTGGCTCCTTCAG |
F-pea3 | AGAAGAACCGTCCAGCCATGA |
R-pea3 | AACATAACGCTCACCAGCCAC |
F-msxb | ACACTTTGTCGAGCGTTTCGG |
R-msxb | TCTTGTGCTTGCGTAAGGTGC |
F-ptch1 | GGGCAGCTAATCTGGAGACGG |
R-ptch1 | GCGCCTCTACGGTCAAAATG |
F-ptch2 | TGCCACGCCGCTTTTGCTTT |
R-ptch2 | GTTTCAATGGCAGCGACCCG |
F-bmp2a | GCAGAGCCAACACTATCAGGAG |
R-bmp2a | CCACTTTAATACAGCAGGAGTTACG |
F-axin2 | GGACACTTCAAGGAACAACTAC |
R-axin2 | CCTCATACATTGGCAGAACTG |
F-cmyc | TAACAGCTCCAGCAGCAGTG |
R-cmyc | GCTTCAAAACTAGGGGACTG |
F-cyclinD1 | GCCAAACTGCCTATACATCAG |
R-cyclinD1 | TGTCGGTGCTTTTCAGGTAC |
F-β-actin2 | GCAGAAGGAGATCACATCCCTGGC |
R-β-actin2 | CATTGCCGTCACCTTCACCGTTC |
F-kcnk5b | ATCACTCTCCTCGTCTGCAACG |
R-kcnk5b | GAGTCCCATGCACAACGTGCAG |
F-GFP | AAGGGCATCGACTTCAAGG |
R-GFP | TGCTTGTCGGCCATGATATAG |