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. 2021 Apr 30;2021:8877540. doi: 10.1155/2021/8877540

Figure 3.

Figure 3

Verification of the existence and expression of human KLK1 and rat KLK1 genes in the prostate. (a) Representative human KLK1 genomic DNA bands in the prostate through conventional PCR followed by agarose gel electrophoresis. (b) Relative mRNA expression of the human KLK1 and rat KLK1 to β-actin within the prostate of different groups by RT-PCR. (c, d) Rat KLK1 and human KLK1 protein expression in the prostate by Western blot. (e) KLK1 protein expression and location in the prostate by IHC (the KLK1 antibody used in this experiment is reactive to both human KLK1 and rat KLK1; magnification ×100). For each group, values are presented as the mean ± SD of 10 rats per group. ∗∗P < 0.01 (aTGR vs. aWTR). ##P < 0.01 (aWTR vs. yWTR). §§P < 0.01 (aTGR vs. yWTR).