Fig. 1. Schematic diagram of NC8-pSIP409-alr-ACEIP construction and expression. (a) Schematic diagram of ACEIP recombinant expression vector. (b) Agarose gel electrophoresis of plasmid. Plasmid digested by restriction enzymes plaNcoI and BamHI (Lane 1) and undigested plasmid (Lane 2) were run on agarose gel and expected bands are shown (5808 bp for vector and 1988 bp for target gene). (c) Western blot determined that ACEIP fusion protein was successfully expressed in NC8-pSIP409-alr. (d) Growth curve of recombinant Lactobacillus plantarum NC8-pSIP409-alr-ACEIP. ACEIP: angiotensin-converting enzyme inhibitory peptide; OD₆₀₀: optical density at 600 nm.