Fig. 10. Effects of NC8-pSIP409-alr-ACEIP on AngII and ACE2 protein expression levels. (a) Protein profiles of AngII, ACE2, and β-actin. β-Actin was used as an internal control, and the induced cells were harvested by centrifugation at 1500 r/min for 3 min at 4 °C and were suspended in 50 mmol/L PBS, followed by sonic disruption. The cell-free extract was analyzed on 15% SDS-PAGE and subjected to western blotting using angiopoietin 2 rabbit polyclonal antibody, ACE2 rabbit polyclonal antibody, and β-actin mouse monoclonal antibody. (b) The ratio of AngII to β-actin. (c) The ratio of ACE2 to β-actin. Results represent the mean±standard deviation (SD) of triplicate assays. ^*** P<0.001, compared with the H₂O₂ positive control group according to ANOVA. ACEIP: angiotensin-converting enzyme inhibitory peptide; SDS-PAGE: sodium dodecylsulfate-polyacrylamide gel electrophoresis; ACE2: angiotensin-converting enzyme 2; ANOVA: analysis of variance.