Fig. 4. Structure determination and model building of the MyD88^TIR higher-order assembly by MicroED and SFX.

Models and maps are presented of the remodelled BB loop (residues 186–204; top) and CD loop (residues 242–251; bottom) for the a MicroED and b SFX^a structures. The carbon atoms in the MicroED and SFX^a structures are shown in grey and green, respectively. Nitrogen, oxygen and sulfur atoms are shown in blue, red and yellow, respectively. The electrostatic scattering potential (MicroED) and electron density (SFX) 2mFo − DFc maps (blue isomesh) are contoured at 1.2σ, and the difference mFo − DFc maps (green and red isomesh for positive and negative density, respectively) are contoured at 2.8σ. No missing reflections were restored using weighted Fc values for map calculations.