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. 2021 May 10;12:2594. doi: 10.1038/s41467-021-22813-w

Fig. 2. BMP and FGF2 condition drives a quiescence reversible state in NSCs.

Fig. 2

a Histogram of cytometry quantification of CD9 in the different conditions MFI (median fluorescence intensity) (n = 4). b Immunostaining of NSCs treated with EGF/FGF, BMP, and BMP/FGF for 3 days. OLIG2 (red), SOX2 (green), GFAP (green) NESTIN (green) KI67 (red) and ID1 (red). Nuclear counterstaining in blue with DRAQ5. Images showing ICC in group of cells to appreciate staining (n = 5). c Quantification of the DNA content using DAPI and flow cytometry (n = 3). d Cytometry analysis of double knock-in mCherry-p27 and eGFP-PCNA NSC line in the different conditions (n = 4). e Quantification of the relative expression of different genes in the cells treated with BMP and BMP/FGF2 (n = 3). f EdU incorporation images (yellow) in the NSCs in presence of EGF/FGF. Nuclear counterstaining with DAPI (blue). Quantification of percentage of EdU positive cells, during the treatment (left), and after to re-exposure to mitogens (right) (n = 3). g (top) Phase contrast images of the colony-forming assay of the cells treated with BMP and BMP/FGF (bottom). Quantification of the number of colonies after to re-exposure to mitogens (n = 5). Scale bar in (b, f, g) is 50 μm. Mean is indicated in the box and whiskers plots from minimum to maximum. Data are shown as mean ± SEM of the indicated number of the experiments (n) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001). Source data are provided as a Data Source File.