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. 2021 May 10;12:2594. doi: 10.1038/s41467-021-22813-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematics of the experiments. NSCs derived from mouse, after several months of expansion in vitro are transplanted in the SVZ for 1 month. b Panoramic pictures of the NSCs (GFP, green) in the SVZ after 1 month. NSCs were treated with EGF/FGF, BMP-4, and FGF/BMP-4 previously to be transplanted. c Immunostaining for GFP (green), GFAP (yellow), Ki67 (red) nuclear counterstaining with DAPI (blue). d Immunostaining for GFP (green), BIII TUBULIN (red), DAPI (blue) showing neurons arriving to the OB. e Immunostaining for GFP (green), BIII tubulin (magenta) and GAD65/67 (red) and nuclear counterstaining with DAPI (blue). LV: Lateral Ventricle. OB: Olfactory Bulb. Detail of co-staining GFP and BIII TUBULIN (red). Scale bar in (b) is 100 μm, (c, d) is 50 μm and in (e) is 20 μm. Scale bar in detail is 20 μm (n = 3 analyzed transplanted mice per condition). Schematics in (a) were generated using BioRender software.