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. 2021 May 10;12:2594. doi: 10.1038/s41467-021-22813-w

Fig. 4. Dormant and primed quiescent NSCs have distinct signaling pathways and transcriptional programs.

Fig. 4

a RPPA data analysis of the NSCs in BMP and BMP/FGF (n = 3). b The top biological GO terms in biological process enriched genes in BMP/FGF condition. c Heatmap showing the top significant genes. d Lrig1 expression by QPCR in the different conditions (n = 3). e Histogram of LRIG1 expression by cytometry (n = 3). Quantifications show mean fluorescent intensity (MFI). f Immunoblot of LRIG1, EGFR (total), and EGFR-P. Loading control GAPDH. g Immunoblot for LRIG1, pAKT, ppERK1/2, GAPDH in NSCs treated with BMP and BMP + FGF for 3 days (n = 3). Different kinases inhibitors were used (Wortmannin, GSK, PD0325901, Tofacitinib) (n = 3). h Immunoblot for SOX2, pSMAD1, SMAD1, GAPDH, ppERK1/2, ERK in NSCs treated with BMP and BMP/FGF for 3 days and re-exposure to EGF + FGF, different time exposures (n = 3). i Immunoblot for Lrig1 in NSCs treated with BMP/FGF and BMP and re-exposure to EGF/FGF in a time course. Loading control, ERK1/2 (n = 3). Data are shown as mean ± SEM of the indicated number of the experiments (n) (∗p < 0.05; ∗∗p < 0.01). Source data are provided as a Source Data File.