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. 2021 May 10;7:98. doi: 10.1038/s41420-021-00471-7

Fig. 4. Effect of hAD-MSCs and hAD-MSC EVs on the immunoinflammatory phenotype of microglia.

Fig. 4

A Representative immunofluorescence images with staining for M1 marker CD11b (green), F-actin phalloidin (white), nuclei with DAPI (blue), and hAD-MSCs labeled with CellBrite (red). Scale bar = 49µm. B Western blots showing the protein expression of inducible nitric oxide (iNOS) and GAPDH in control and stimulated (with LPS) conditions when HMC3 cells were co-cultured alone or with hAD-MSCs or hAD-MSCs EVs. C Quantification of the Western Blot bands shown above. NA indicates a not applicable value due to limited signal detection for GAPDH.