Table 3.
Odds ratios and confidence intervals for metabolites and breast cancer risk in the E3N cohort.
| Plasma metabolite or metabolite groupa | 1D NMR 1H chemical shifts (ppm) | Study group | Scaled mean intensity (SD) in controlsb | Scaled mean intensity (SD) in casesb | Odds ratio (95% CI) per SD increase in plasma concentrationc | Odds ratio (FDR-adjusted CI) per SD increasec | Raw P-value | False discovery rate P-value |
|---|---|---|---|---|---|---|---|---|
| N-acetyl glycoproteins (Acetylation of proteins) | 2.04, 2.07 | All women | 99.6 (8.4) | 100.4 (8.3) | 1.17 (1.05–1.31) | – | 0.004 | 0.162 |
| Post-menopausal | 99.4 (8.5) | 99.78 | 1.09 (0.96–1.24) | – | 0.168 | 0.844 | ||
| Pre-menopausal | 100.1 (8.1) | 102.89 | 1.53 (1.19–1.96) | 1.53 (1.11–2.11) | 0.001 | 0.015 | ||
| Pre-menopausal, adjusted ethanol | 100.1 (8.1) | 102.89 | 1.43 (1.09–1.89) | – | 0.011 | 0.194 | ||
| Ethanol (Alcohol) | 1.17 | All women | 99.9 (33.6) | 100.1 (31.1) | 1.14 (1.02–1.27) | – | 0.021 | 0.351 |
| Post-menopausal | 100.7 (37.9) | 99.5 (30.1) | 1.05 (0.93–1.20) | – | 0.409 | 0.913 | ||
| Pre-menopausal | 97.4 (8.1) | 102.1 (34.4) | 1.44 (1.13–1.83) | 1.44 (1.05–1.97) | 0.004 | 0.03 | ||
| Histidine (Amino acid) | 3.16, 3.23, | All women | 99.4 (18.4) | 100.6 (18.3) | 1.10 (0.98–1.23) | – | 0.1 | 0.535 |
| 7.04, 7.73 | Post-menopausal | 99.6 (18.1) | 99.5 (18.2) | 0.97 (0.85–1.10) | – | 0.615 | 0.973 | |
| Pre-menopausal | 98.6 (19.4) | 104.2 (18.2) | 1.70 (1.29–2.23) | 1.70 (1.19–2.41) | <0.001 | 0.006 | ||
| Pre-menopausal, adjusted ethanol | 98.6 (19.4) | 104.2 (18.2) | 1.58 (1.18–2.12) | – | 0.002 | 0.08 | ||
| Glycerol (Alcohol) | 3.56, 3.65 | All women | 99.7 (13.7) | 100.3 (13.8) | 1.07 (0.96–1.19) | – | 0.217 | 0.767 |
| Post-menopausal | 99.3 (14.3) | 98.9 (13.2) | 0.97 (0.86–1.10) | – | 0.649 | 0.973 | ||
| Pre-menopausal | 101.3 (11.4) | 105.2 (14.7) | 1.55 (1.19–2.02) | 1.55 (1.11, 2.18) | 0.001 | 0.015 | ||
| Pre-menopausal, adjusted ethanol | 101.3 (11.4) | 105.2 (14.7) | 1.40 (1.04–1.89) | – | 0.025 | 0.194 | ||
| Ornithine (Amino acid) | 1.82 | All women | 99.8 (11.3) | 100.2 (11.2) | 1.07 (0.96–1.19) | – | 0.2 | 0.767 |
| Post-menopausal | 100.1 (11.6) | 99.7 (11.4) | 0.99 (0.87–1.12) | – | 0.882 | 0.973 | ||
| Pre-menopausal | 99 (10.2) | 101.9 (10.5) | 1.43 (1.13–1.82) | 1.43 (1.06–1.95) | 0.003 | 0.03 | ||
| Pre-menopausal, adjusted ethanol | 99 (10.2) | 101.9 (10.5) | 1.30 (1.00–1.70) | – | 0.052 | 0.194 | ||
| Leucine (Amino acid) | 1.70, 1.71, 3.72 | All women | 99.6 (9.5) | 100.4 (10.3) | 1.06 (0.96–1.18) | – | 0.262 | 0.767 |
| Post-menopausal | 99.6 (9.7) | 100 (10.6) | 1.00 (0.89–1.12) | – | 0.959 | 0.973 | ||
| Pre-menopausal | 99.6 (8.9) | 101.7 (9.2) | 1.37 (1.08–1.74) | 1.37 (1.01–1.86) | 0.009 | 0.046 | ||
| Pre-menopausal, adjusted ethanol | 99.6 (8.9) | 101.7 (9.2) | 1.28 (1.00–1.64) | – | 0.051 | 0.194 | ||
| Albumin (Protein) | 2.97 | All women | 99.913 | 100.113 | 1.04 (0.93–1.17) | – | 0.468 | 0.767 |
| Post-menopausal | 99.1 (13.2) | 98.5 (12.7) | 0.95 (0.83–1.08) | – | 0.425 | 0.913 | ||
| Pre-menopausal | 102.3 (12.2) | 105.7 (12.4) | 1.39 (1.09–1.78) | 1.39 (1.02–1.91) | 0.008 | 0.046 | ||
| Pre-menopausal, adjusted ethanol | 102.3 (12.2) | 105.7 (12.4) | 1.30 (1.00–1.70) | – | 0.054 | 0.194 | ||
| Glutamine (Amino acid) | 2.1, 2.13, 2.43, 2.46 | All women | 99.9 (12.6) | 100.1 (12.7) | 1.03 (0.92–1.15) | – | 0.632 | 0.767 |
| Post-menopausal | 99.7 (12.5) | 99.1 (12.6) | 0.94 (0.82–1.06) | – | 0.314 | 0.913 | ||
| Pre-menopausal | 100.5 (12.8) | 103.7 (12.2) | 1.33 (1.07–1.67) | 1.33 (1.00–1.78) | 0.012 | 0.049 | ||
| Pre-menopausal, adjusted ethanol | 100.5 (12.8) | 103.7 (12.2) | 1.29 (1.02–1.63) | – | 0.035 | 0.194 | ||
| Glutamate (Amino acid) | 2.04, 2.12, 2.34, 3.75 | All women | 99.8 (10.9) | 100.2 (10.8) | 1.03 (0.93–1.15) | – | 0.557 | 0.767 |
| Post-menopausal | 99.6 (10.9) | 99.2 (10.8) | 0.94 (0.83–1.07) | – | 0.364 | 0.913 | ||
| Pre-menopausal | 100.8 (10.9) | 103.3 (10.1) | 1.34 (1.07–1.68) | 1.34 (1.00–1.79) | 0.01 | 0.049 | ||
| Pre-menopausal, adjusted ethanol | 100.8 (10.9) | 103.3 (10.1) | 1.30 (1.02–1.66) | – | 0.032 | 0.194 | ||
| Pyruvate (Organic acid) | 2.36 | All women | 99.6 (13.6) | 100.4 (14.6) | 1.03 (0.92–1.15) | – | 0.579 | 0.767 |
| Post-menopausal | 99.3 (13.4) | 98.9 (13.4) | 0.94 (0.82–1.07) | – | 0.331 | 0.913 | ||
| Pre-menopausal | 100.7 (14.2) | 105.4 (17.4) | 1.42 (1.11–1.81) | 1.42 (1.04–1.94) | 0.005 | 0.036 | ||
| Pre-menopausal, adjusted ethanol | 100.7 (14.2) | 105.4 (17.4) | 1.33 (1.03–1.73) | – | 0.028 | 0.194 |
aOnly metabolites whose FDR-adjusted P-values fell beneath the significance threshold of 0.05 in one of the study groups are tabulated.
bWhere a value of 100 is attributed to the mean intensity in all participants.
cModels were adjusted for smoking status, diabetes status, BMI, waist to hip ratio, daily alcohol intake, duration of hormone treatment at blood collection, waiting time before plasma fractionation and biobank storage time.