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. 2021 May 10;11:9838. doi: 10.1038/s41598-021-89288-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mechanical strain regulates gene transcription of cell adhesion, drug metabolism and proliferation genes. MDA-MB-231 breast cancer cells were treated with mechanical strain for 24 h at 0, 7.5, and 15% maximal strain at a frequency of 1 Hz. Total RNA was isolated and RNA sequencing was performed (n = 4). (A) Hierarchical clustering of the most significantly regulated genes. FPKM = fragments per kilobase of transcript per million mapped reads. (B) Venn diagrams for significantly regulated genes in the 7.5% and 15% mechanical strain groups. (C) The top five most upregulated and downregulated gene ontology groups for cells treated with 7.5% strain. (D) The top five most upregulated and downregulated gene ontology groups for cells treated with 15% strain. R software was used to create parts of this figure²².