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. 2021 May 10;11:9838. doi: 10.1038/s41598-021-89288-z

Figure 5.

Figure 5

Strain differentially activates Smad2/3 and Yap/Taz in breast cancer and epithelial cells. (A) MDA-MB-231 cells were treated with mechanical strain for 24 h at 1 Hz with varying magnitudes of maximal strain. Immunostaining for phospho-Smad2/3, Smad2/3 and Yap/Taz was performed. Bar = 100 μm. (B) Quantification of total Smad2/3 in the cells (n = 20). *p < 0.05 versus static group (n = 20). (C) Quantification of phosphorylated Smad2/3 in cells treated mechanical load (n = 20). *p < 0.05 versus the static group for the same subcellular location. (D) Quantification of nuclear to cytoplasmic Yap/Taz staining (n = 20). *p < 0.05 versus static group. (E-J) Western blotting for lysates from cells treated with mechanical load for 24 h and inhibitors Lck, PI3K, Syk and Yap (Vert; verteporfin). Full-length blots/gels are presented in Supplemental Figs. S12–S17.