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. 2021 May 10;11:9838. doi: 10.1038/s41598-021-89288-z

Figure 7.

Figure 7

Mechanical loading decreases tumor growth and increases metastasis to the skull in immune compromised mice. (A) Luciferase expressing MDA-MB-231 cells were grown under static or mechanically loaded conditions for 24 h and then implanted into the mammary fat pad of nu/nu mice. Radiance of mice with orthotopic tumor implantation at 22 days. (B) Quantification of the radiance in the mammary fat pad. *p < 0.05 versus static group (n = 10). (C) Laser speckle imaging of mice with orthotopic implantation of MDA-MB-231 cells after mechanical conditioning. (D) Ratio of perfusion of the mammary fat pad with tumor implantation to contralateral control fat pad. *p < 0.05 between indicated groups (n = 10). (E) Images of luminescence as measured by IVIS for nu/nu given an intravenous injection of MDA-MB-231 cells. The cells were cultured under static or 7.5% strain for 24 h prior to injection. (F) Quantification of luminescence in the head of the mice given an intravenous injection of MDA-MB-231 cells at day 42 following injection. *p < 0.05 versus static group (n = 8–9). (G) Luminescence in the heads of NOD Scid mice 42 days after tail vein injection of MDA-MB-231 cells. (H) Quantification of the luminescence in the head of the mice after 42 days. *p < 0.05 versus indicated group (n = 7–8).