Fig. 4. Subcompartment repositioning between normal and cancer cell lines.

a Schematic comparison of the comparison between normal and cancer cell lines from breast (red), prostate (blue), and pancreas (purple) tissues. b Compartment (color coded) and domain rank (Y-axis) of the FOXO1-containing domain in normal and cancer cells. c H3K27ac intensity at the FOXO1 locus for normal and cancer cell lines derived from breast (red), prostate (blue), and pancreatic (purple) tissues. d, e FOXO1 mRNA expression level (log2-transformed FPKM normalized RNA-seq reads) in either the cell lines with matching Hi-C data (d), or in normal and cancer samples profiled by The Cancer Genome Atlas (TCGA) (e). Each data point represents a replicate in (d, n = 3, 1, 3, 1–4, 3, 1–4, 1, 1, 1, 2) and a sample in (e, numbers of samples in brackets). f Schematic representation of interdomain interactions (dashed lines) between the FOXO1-containing compartment domain (yellow) and regions that do not change compartment in normal and cancer cells (gray). The FOXO1-domain is repositioned in cancer cells. g Distance normalized contact values between FOXO1 and regions that do not change compartment in the compared breast (left, n = 7, 7, 4, 2, 5, 5, 7, 8), prostate (center, n = 11, 10, 9, 6, 3, 6, 7, 6), and pancreatic (right, n = 9, 12, 4, 5, 6, 8, 16, 6) cell lines. The bounds of the box in the plot are first quartile (Q₁) and third quartile (Q₃). The lower and upper whiskers are computed by extending the box bounds by 1.5*IQR, where IQR = Q₃ − Q₁.