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. 2021 May 10;12:2603. doi: 10.1038/s41467-021-22742-8

Fig. 2. Levels of immunoreactivity for excitatory and inhibitory synaptic proteins are reduced in AD and DS.

Fig. 2

a, c, e, g Plots showing the proportion of PSD-95+ and gephyrin (GPHN) + puncta in each layer with immunofluorescence in low (<75), mid (75–90), or high (>90) intensity ranges for each group (group means ± SEM; based on Fig. 1e-h IR (Immunoreactivity) intensity distributions). Each dot represents the value for one subject. RM-ANOVA (group × intensity interaction) indicated a significant effect for layer 2 only: P = 0.0019 for PSD-95 and P = 0.0133 for GPHN. RM-ANOVA for layer 1, P = 0.1027 for PSD-95 and P = 0.4151 for GPHN (n = 5 controls, 5 AD and 6 DS). b, d, f, h Box plots show the ratio of high-to-low labeling intensities for each individual case, the median is represented by the line within the box, and the first and third quartiles are represented by the ends of the box. The whiskers extend from each end of the box to the first or third quartile ±1.5 (interquartile range). As shown, all AD and DS cases exhibited significantly lower high-to-low intensity ratios as compared to controls for both postsynaptic proteins in each layer: PSD-95 layer 1, (F (2,13) = 14.17, P < 0.0005); GPHN layer 1 (F (2,13) = 5.1, P = 0.023); PSD-95 layer 2, (F (2,13) = 8.4, P = 0.0045); GPHN layer 2 (F (2,13) = 37.54, P < 0.0001). P values shown are from the two-sided Dunnett’s post hoc test comparing AD or DS to control.