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. 2021 May 10;4:544. doi: 10.1038/s42003-021-02054-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, c, e, g mRNA expression of ACTRT1 during differentiation of keratinocytes, NHEK (a N = 5) and HaCaT (c N = 3), and epithelial cells, ARPE19 (e N = 3) and hTERT-RPE1 (g N = 5). Data are presented as means of the fold change compared to the value of undifferentiated samples. Each open circle represents one independent experiment. b, d, f, h Representative images of ARP-T1 expression during differentiation of keratinocytes, NHEK (b) and HaCaT (d), and epithelial cells, ARPE19 (f) and hTERT-RPE1 (h). ARP-T1 was detected using guinea pig anti-ARP-T1 antisera (b) or mouse antibody (d, f, h). * indicates polymers of ARP-T1 confirmed by mass spectrometry analysis. Keratin 10 and IFT88 were used as markers of cell differentiation in keratinocytes and epithelial cells, respectively, actin and tubulin as loading controls.