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. 2021 May 10;4:544. doi: 10.1038/s42003-021-02054-9

Fig. 3. ARP-T1 interacts with proteins involved in ciliary machinery.

Fig. 3

a, b HeLa (a) and hTERT-RPE1 (b) cells were transduced with lentiviral vectors, empty vector (V), ACTRT1 mutant (M) and ACTRT1 WT (WT), and immunoprecipitated (IP) with anti-FLAG monoclonal antibody M2-conjugated agarose, and analyzed by immunoblot with indicated antisera. c Immunofluorescence stainings of ARP-T1, acetylated-tubulin and rootletin in 35 days of serum-starved ARPE19 cells. Nuclei are stained with DAPI. Scale bar, 5 µm. Higher magnifications of the boxed area are shown on right three panels. Scale bar, 1 µm. d Immunofluorescence staining of ARP-T1, gamma-tubulin, EHD4, and septin 2 in 48 h of serum-starved hTERT-RPE1 cells. Nuclei are stained with DAPI. Scale bar, 5 µm. Higher magnifications of the boxed area are shown on the right three panels. Scale bar, 1 µm.