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. Author manuscript; available in PMC: 2021 May 15.
Published in final edited form as: ACS Infect Dis. 2020 Nov 11;7(5):1126–1142. doi: 10.1021/acsinfecdis.0c00634

Figure 5.

Figure 5.

Intestinal MUC2 influences C. difficile gene expression C. difficile R20291 was inoculated in the fully defined minimal medium CDMM with glucose, mannose, 1 mg/mL human LS174T MUC2, and/or A. muciniphila BAA-835, B. thetaiotaomicron ATCC 29148, and R. torques ATCC 2775. After 16 h of incubation, samples were collected for RNA isolation and qPCR analysis. qPCR analysis of adhesion- related genes, (A) splA and (B) cwp84 or flagella-related genes (C) flgG and (D) fliC. (E) Chemotaxis analysis of CFDA-SE labeled C. difficile toward buffer controls, 1 mg/mL MUC2 (LS174T), or supernatant from A. muciniphila, B. thetaiotaomicron, or R. torques incubated with MUC2 in PBS for 2 h. n = 6 replicates, repeated three independent times. *p < 0.05, One-Way ANOVA.