Fig. 1.
Basic characterization of C60-OO preparations. a Photographs of commercial C60-OO preparations as received from the manufacturer in comparison to pristine C60-OO. b Representative HPLC chromatograms of extracted C60 from C60-OO preparations. Inset is zoomed to aid in visualization of impurity peaks. Note the decreased peak height of the main C60 peak and increased non-C60 impurity peaks seen in the commercially sourced C60-OO. c Measured C60 concentrations in C60-OO samples relative to their label claims. In-house C60-OO is shown without a labeled concentration. Data are mean ± SEM. d ROS protection activity of C60-OO preparations as measured by 4-NBP assay. Only in-house pristine C60-OO exhibits protection. Data are mean ± SEM. Data were analyzed with one-way ANOVA with Bonferroni post-hoc correction. ***p < 0.001, ****p < 0.0001, ns = not significant